Sizes of these terms reflect their relevance to your search.
A specific substrate, M13 DNA:RNA-[32P]DNA, was synthesized to investigate the mode of cleavage of enzymes with RNase H activity. RNase H(70) from Saccharomyces cerevisiae hydrolyzes the phosphodiester bond at the RNA-DNA junction of this substrate, thereby producing a 5'-monophosphate-terminated polydeoxyribonucleotide and 3'-hydroxyl-terminated oligoribonucleotides.
Citation
R Karwan, U Wintersberger. Yeast ribonuclease H(70) cleaves RNA-DNA junctions. FEBS letters. 1986 Oct 6;206(2):189-92
Mesh Tags
Substances
PMID: 2428664
View Full Text
