Crystallization of the C-terminal domain of the fibre protein from snake adenovirus 1, an atadenovirus.

Abhimanyu K Singh, Rosa Menéndez-Conejero, Carmen San Martín, Mark J van Raaij

Acta crystallographica. Section F, Structural biology and crystallization communications 2013 Dec

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Adenovirus fibre proteins play an important role in determining viral tropism. The C-terminal domain of the fibre protein from snake adenovirus type 1, a member of the Atadenovirus genus, has been expressed, purified and crystallized. Crystals were obtained belonging to space groups P2(1)2(1)2(1) (two different forms), I2(1)3 and F23. The best of these diffracted synchrotron radiation to a resolution of 1.4 Å. As the protein lacks methionines or cysteines, site-directed mutagenesis was performed to change two leucine residues to methionines. Crystals of selenomethionine-derivatized crystals of the I2(1)3 form were also obtained and a multi-wavelength anomalous dispersion data set was collected.

Citation

Abhimanyu K Singh, Rosa Menéndez-Conejero, Carmen San Martín, Mark J van Raaij. Crystallization of the C-terminal domain of the fibre protein from snake adenovirus 1, an atadenovirus. Acta crystallographica. Section F, Structural biology and crystallization communications. 2013 Dec;69(Pt 12):1374-9