Effects of oligoelements Se, Zn, and Mn plus Lachesis muta venom in experimental scleroderma.

Biological trace element research 2014 Feb

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Scleroderma, sclerosis of the skin, is a severe autoimmune disease refractant to all kind of treatments. To study the in vivo effects of a combination of three oligoelements selenium (Se), zinc (Zn), and manganese (Mn) plus Lachesis muta veno m (O-LM) on the bleomycin (BLM)-induced scleroderma mouse experimental model. C3H mice were randomly divided into four groups: control (phosphate-buffered saline (PBS)), O-LM, BLM, and BLM + O-LM. All administrations were performed subcutaneously into the back of mice. BLM was injected 5 days per week for three consecutive weeks and O-LM was administered simultaneously with BLM from the beginning of the experiments and lasted for 3 weeks after the final BLM or PBS injection (for O-LM and BLM + O-LM groups), when animals were sacrificed and histopathological, immunohistochemical, thiobarbituric acid reactive species (TBARS) evaluation, and autoantibodies detection were determined. O-LM significantly reduced BLM-induced enhanced dermal thickness (605 ± 47 vs. 956 ± 59 μm, P < 0.01), collagen deposition, and mast cells infiltration (43.1 ± 1.0 vs. 102 ± 14.1 mast cells, P < 0.05). O-LM administration significantly blocked BLM-induced oxidative damage and the enhanced immunoreactive fibroblasts for α-smooth muscle acti n while reduced BLM-induced autoantibodies that strongly react mainly with skin and spleen. O-LM significantly reduced BLM-induced scleroderma through the modulation of antioxidant and immunological pathways.

Citation

Ernesto J V Crescenti, Vanina A Medina, Lorena A Sambuco, Graciela A Cremaschi, Ana M Genaro, Graciela P Cricco, Gabriela A Martín, Eduardo Valli, Diego J Martinel Lamas, Juan C Perazzo, Elena S Rivera, Rosa M Bergoc. Effects of oligoelements Se, Zn, and Mn plus Lachesis muta venom in experimental scleroderma. Biological trace element research. 2014 Feb;157(2):138-46