TIAM2 enhances non-small cell lung cancer cell invasion and motility.

TIAM2, a Rac guanine nucleotide exchange factor, is closely associated with cell adherence and migration. Here, we aimed to investigate the role of TIAM2 in non-small cell lung cancer (NSCLC) cells. A small interference RNA (siRNA) was introduced to silence the expression of TIAM2. Invasion and motility assays were then performed to assess the invasion and motility potential of NSCLC cells. GST-pull down assays were used to detect activation of Rac1. TIAM2 was highly expressed in NSCLC cells. Knockdown of TIAM2 inhibited the invasion and motility, and suppressed activation of Rac1. Further experiments demonstrated that knockdown of TIAM2 could up-regulate the expression of E-cadherin, and down- regulate the expression of MMP-3, Twist and Snail. Our data suggest that TIAM2 can promote invasion and motility of NSCLC cells. Activation of Rac1 and regulation of some EMT/invasion-related genes may be involved in the underlying processes.

Citation

Zheng-Yuan Zhao, Chen-Guang Han, Jun-Tao Liu, Chang-Lei Wang, Yi Wang, Li-Ya Cheng. TIAM2 enhances non-small cell lung cancer cell invasion and motility. Asian Pacific journal of cancer prevention : APJCP. 2013;14(11):6305-9

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PMID: 24377522

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