Categorisation of sugar acid dehydratases in Aspergillus niger.

Francine A Motter, Joosu Kuivanen, Hanna Keränen, Satu Hilditch, Merja Penttilä, Peter Richard

Fungal genetics and biology : FG & B 2014 Mar

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In the genome of Aspergillus niger five genes were identified coding for proteins with homologies to sugar acid dehydratases. The open reading frames were expressed in Saccharomyces cerevisiae and the activities tested with a library of sugar acids. Four genes were identified to code for proteins with activities with sugar acids: an l-galactonate dehydratase (gaaB), two d-galactonate dehydratases (dgdA, dgdB) and an l-rhamnonate dehydratase (lraC). The specificities of the proteins were characterised. The l-galactonate dehydratase had highest activity with l-fuconate, however it is unclear whether the enzyme is involved in l-fuconate catabolism. None of the proteins showed activity with galactaric acid or galactarolactone. Copyright © 2013 Elsevier Inc. All rights reserved.

Citation

Francine A Motter, Joosu Kuivanen, Hanna Keränen, Satu Hilditch, Merja Penttilä, Peter Richard. Categorisation of sugar acid dehydratases in Aspergillus niger. Fungal genetics and biology : FG & B. 2014 Mar;64:67-72