Light-regulated tetracycline binding to the Tet repressor.

Jayoung Moon, Jongsik Gam, Seung-Goo Lee, Young-Ger Suh, Jeeyeon Lee

Chemistry (Weinheim an der Bergstrasse, Germany) 2014 Feb 24

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Elucidation of the signal-transmission pathways between distant sites within proteins is of great importance in medical and bioengineering sciences. The use of optical methods to redesign protein functions is emerging as a general approach for the control of biological systems with high spatiotemporal precision. Here we report the detailed thermodynamic and kinetic characterization of novel chimeric light-regulated Tet repressor (TetR) switches in which light modulates the TetR function. Light absorbed by flavin mononucleotide (FMN) generates a signal that is transmitted to As-LOV and YtvA-LOV fused TetR proteins (LOV=light-oxygen-voltage), in which it alters the binding to tetracycline, the TetR ligand. The engineering of light-sensing protein modules with TetR is a valuable tool that deepens our understanding of the mechanism of signal transmission within proteins. In addition, the light-regulated changes of drug binding that we describe here suggest that engineered light-sensitive proteins may be used for the development of novel therapeutic strategies. Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Citation

Jayoung Moon, Jongsik Gam, Seung-Goo Lee, Young-Ger Suh, Jeeyeon Lee. Light-regulated tetracycline binding to the Tet repressor. Chemistry (Weinheim an der Bergstrasse, Germany). 2014 Feb 24;20(9):2508-14