Erythrocyte-binding studies on an acidic lectin from winged bean (Psophocarpus tetragonolobus).

S R Patanjali, S U Sajjan, A Surolia

UGC Centre for Advanced Studies, Indian Institute of Science, Bangalore.

The Biochemical journal 1988 Jun 15

filter terms:

An acidic lectin (WBA II) was isolated to homogeneity from the crude seed extract of the winged bean (Psophocarpus tetragonolobus) by affinity chromatography on lactosylaminoethyl-Bio-Gel. Binding of WBA II to human erythrocytes of type-A, -B and -O blood groups showed the presence of 10(5) receptors/cell, with high association constants (10(6)-10(8) M-1). Competitive binding studies with blood-group-specific lectins reveal that WBA II binds to H- and T-antigenic determinants on human erythrocytes. Affinity-chromatographic studies using A-, B-, H- and T-antigenic determinants coupled to an insoluble matrix confirm the specificity of WBA II towards H- and T-antigenic determinants. Inhibition of the binding of WBA II by various sugars show that N-acetylgalactosamine and T-antigenic disaccharide (Thomsen-Friedenreich antigen, Gal beta 1-3GalNAc) are the most potent mono- and di-saccharide inhibitors respectively. In addition, inhibition of the binding of WBA II to erythrocytes by dog intestine H-fucolipid prove that the lectin binds to H-antigenic determinant.

Citation

S R Patanjali, S U Sajjan, A Surolia. Erythrocyte-binding studies on an acidic lectin from winged bean (Psophocarpus tetragonolobus). The Biochemical journal. 1988 Jun 15;252(3):625-31