Identification of PLP2 and RAB5C as novel TPD52 binding partners through yeast two-hybrid screening.

Hamideh Shahheydari, Sarah Frost, Brian J Smith, Guy E Groblewski, Yuyan Chen, Jennifer A Byrne

Molecular biology reports 2014 Jul

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Tumor protein D52 (TPD52) is overexpressed in different cancers, but its molecular functions are poorly defined. A large, low-stringency yeast two-hybrid screen using full-length TPD52 bait identified known partners (TPD52, TPD52L1, TPD52L2, MAL2) and four other preys that reproducibly bound TPD52 and TPD52L1 baits (PLP2, RAB5C, GOLGA5, YIF1A). PLP2 and RAB5 interactions with TPD52 were confirmed in pull down assays, with interaction domain mapping experiments indicating that both proteins interact with a novel binding region of TPD52. This study provides insights into TPD52 functions, and ways to maximise the efficiency of low-stringency yeast two-hybrid screens.

Citation

Hamideh Shahheydari, Sarah Frost, Brian J Smith, Guy E Groblewski, Yuyan Chen, Jennifer A Byrne. Identification of PLP2 and RAB5C as novel TPD52 binding partners through yeast two-hybrid screening. Molecular biology reports. 2014 Jul;41(7):4565-72