Correlation Engine 2.0
Clear Search sequence regions


Sizes of these terms reflect their relevance to your search.

DNA methylation plays an important role in regulating cell growth and disease development. Methylation profiles are examined by bisulfite conversion; however, the lack of markers for bisulfite conversion efficiency and appropriate internal control genes remains a major challenge. To address these issues, we utilized two bioinformatics approaches, coefficients of variances and resampling tests, to identify probes showing stable methylation levels from several independent microarray datasets. Mass spectrometry validated the consistently high methylation levels of the five probes (N4BP2, EGFL8, CTRB1, TSPAN3, and ZNF690) in 13 human tissue types from 24 cell lines. Linear associations between detected methylation levels and methyl concentrations of DNA samples were further demonstrated in three genes (N4BP2, EGFL8, and CTRB1). To summarize, we identified five genes which may serve as internal controls for methylation studies by analyzing large-scale microarray data, and three of them can be used as markers for evaluating the efficiency of bisulfite conversion.

Citation

Tzu-Pin Lu, Kevin T Chen, Mong-Hsun Tsai, Kuan-Ting Kuo, Chuhsing Kate Hsiao, Liang-Chuan Lai, Eric Y Chuang. Identification of genes with consistent methylation levels across different human tissues. Scientific reports. 2014 Mar 12;4:4351

Expand section icon Mesh Tags

Expand section icon Substances


PMID: 24619003

View Full Text