The type II Ca2+/calmodulin-dependent protein kinases are involved in the regulation of cell wall integrity and oxidative stress response in Candida albicans.

Xiaohui Ding, Qilin Yu, Bing Zhang, Ning Xu, Chang Jia, Yijie Dong, Yulu Chen, Laijun Xing, Mingchun Li

Biochemical and biophysical research communications 2014 Apr 18

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The type II Ca(2+)/calmodulin-dependent protein kinases (CaMKs) are thought to play a vital role in cellular regulation in mammalian cells. Two genes CMK1 and CMK2 in the Candida albicans genome encode homologues of mammalian CaMKs. In this work, we constructed the cmk1Δ/Δ, the cmk2Δ/Δ and the cmk1Δ/Δcmk2Δ/Δ mutants and found that CaMKs function in cell wall integrity (CWI) and cellular redox regulation. Loss of either CMK1 or CMK2, or both resulted in increased expression of CWI-related genes under Calcofluor white (CFW) treatment. Besides, CaMKs are essential for the maintenance of cellular redox balance. Disruption of either CMK1 or CMK2, or both not only led to a significant increase of intracellular ROS levels, but also led to a decrease of the mitochondrial membrane potential (MMP), suggesting the important roles that CaMKs play in the maintenance of the mitochondrial function. Copyright © 2014 Elsevier Inc. All rights reserved.

Citation

Xiaohui Ding, Qilin Yu, Bing Zhang, Ning Xu, Chang Jia, Yijie Dong, Yulu Chen, Laijun Xing, Mingchun Li. The type II Ca2+/calmodulin-dependent protein kinases are involved in the regulation of cell wall integrity and oxidative stress response in Candida albicans. Biochemical and biophysical research communications. 2014 Apr 18;446(4):1073-8