Determination of 10-chloromethyl-11-demethyl-12-oxo-calanolide A in rat plasma using liquid chromatography-tandem mass spectrometry and its application to pharmacokinetics.

Li Sheng, Qinghao Zhang, Zhihao Liu, Xin Liu, Baolian Wang, Hui Chen, Yan Li

Biomedical chromatography : BMC 2014 Dec

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A rapid and specific liquid chromatography-tandem mass spectrometry for the quantitation of 10-chloromethyl-11-demethyl-12-oxo-calanolide A (F18), a small-molecule nonnucleoside reverse transcriptase inhibitor, was developed and validated in rat plasma. F18 was monitored by positive electrospray ionization in the selected reaction monitoring mode. The standard curve was linear over the range of 2-1000 ng/mL. The method was used to determine the plasma concentration of F18 after a single oral dose of 50 mg/kg in rats. Copyright © 2014 John Wiley & Sons, Ltd.

Citation

Li Sheng, Qinghao Zhang, Zhihao Liu, Xin Liu, Baolian Wang, Hui Chen, Yan Li. Determination of 10-chloromethyl-11-demethyl-12-oxo-calanolide A in rat plasma using liquid chromatography-tandem mass spectrometry and its application to pharmacokinetics. Biomedical chromatography : BMC. 2014 Dec;28(12):1589-91