Genome-wide binding of MBD2 reveals strong preference for highly methylated loci.

Roberta Menafra, Arie B Brinkman, Filomena Matarese, Gianluigi Franci, Stefanie J J Bartels, Luan Nguyen, Takashi Shimbo, Paul A Wade, Nina C Hubner, Hendrik G Stunnenberg

PloS one 2014

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MBD2 is a subunit of the NuRD complex that is postulated to mediate gene repression via recruitment of the complex to methylated DNA. In this study we adopted an MBD2 tagging-approach to study its genome wide binding characteristics. We show that in vivo MBD2 is mainly recruited to CpG island promoters that are highly methylated. Interestingly, MBD2 binds around 1 kb downstream of the transcription start site of a subset of ∼ 400 CpG island promoters that are characterized by the presence of active histone marks, RNA polymerase II (Pol2) and low to medium gene expression levels and H3K36me3 deposition. These tagged-MBD2 binding sites in MCF-7 show increased methylation in a cohort of primary breast cancers but not in normal breast samples, suggesting a putative role for MBD2 in breast cancer.

Citation

Roberta Menafra, Arie B Brinkman, Filomena Matarese, Gianluigi Franci, Stefanie J J Bartels, Luan Nguyen, Takashi Shimbo, Paul A Wade, Nina C Hubner, Hendrik G Stunnenberg. Genome-wide binding of MBD2 reveals strong preference for highly methylated loci. PloS one. 2014;9(6):e99603