Correlation Engine 2.0
Clear Search sequence regions

  • anti- antibodies (1)
  • anti- igg (1)
  • antibodies (2)
  • antigens (2)
  • blood (1)
  • cohort (1)
  • diagnosis (2)
  • elisa (9)
  • essential (1)
  • female (1)
  • immunoglobulin g (2)
  • lesions skin (1)
  • m stage (1)
  • odds ratio (1)
  • pregnancy (1)
  • probabilities (1)
  • serum (1)
  • spp (3)
  • stand up (1)
  • status (1)
  • Sizes of these terms reflect their relevance to your search.

    The objective of this longitudinal study was to evaluate the immune response against Treponema spp. infection in dairy heifers affected with digital dermatitis (DD). In addition, the accuracy of an indirect ELISA detecting anti-Treponema IgG antibodies in identifying clinical DD status has been assessed. A cohort of 688 pregnant Holstein heifers was evaluated at least 3 times before calving during a period of 6 mo. Complete clinical assessment of DD presence on the back feet of each heifer and blood extraction were performed in a stand-up chute. Digital dermatitis cases were characterized by the M-stage classification system and size and level of skin proliferation. An ELISA was performed on blood serum samples obtained from a subcohort of 130 heifers. For description purposes, the animals were classified by the number of clinical cases experienced during the study period as type I (no clinical cases were observed), type II (only 1 acute clinical case diagnosed), and type III (at least 2 acute clinical cases diagnosed). Multivariable repeated-measures models were used to evaluate the immune response against Treponema spp. infection. A binormal Bayesian model for the ELISA data without cut-point values was used to assess the accuracy of the ELISA as a diagnostic tool. Animals that never experienced a DD event throughout the study kept a constant low level of antibody titer. A 56% increase in mean ELISA titer was observed in heifers upon a first clinical DD case diagnosis. After topical treatment of an acute DD case with oxytetracycline, the antibody titer decreased progressively in type II heifers, achieving mean levels of those observed in healthy cows after a mean of 223 d. Surprisingly, antibody titer was not increased in the presence of M1 (DD lesion <20mm in diameter surrounded by healthy skin) and M4.1 (DD lesion <20mm in diameter embedded in a circumscribed dyskeratotic or proliferative skin alteration) DD stages. Type III cows showed a slight increase in antibody levels. The presence of skin proliferation at first DD diagnosis was found to be associated with an odds ratio of 2.04 of becoming a type III heifer in relation to heifers presenting first lesions without skin proliferation. The ELISA validity was estimated by an area under the curve of 0.88. Predicted probabilities of infection are provided for a range of ELISA values and prevalence of infection. Early detection and treatment is essential to control DD and the ELISA can be used in understanding the immunopathology of DD and shows great promise for prescreening purposes during DD management programs in combination with traditional clinical inspection. Copyright © 2014 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.


    A Gomez, K S Anklam, N B Cook, J Rieman, K A Dunbar, K E Cooley, M T Socha, D Döpfer. Immune response against Treponema spp. and ELISA detection of digital dermatitis. Journal of dairy science. 2014;97(8):4864-75

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 24931522

    View Full Text