An improved and reproducible protocol for the extraction of high quality fungal RNA from plant biomass substrates.

Aleksandrina Patyshakuliyeva, Miia R Mäkelä, Outi-Maaria Sietiö, Ronald P de Vries, Kristiina S Hildén

Fungal genetics and biology : FG & B 2014 Nov

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Isolation of high quantity and quality RNA is a crucial step in the detection of meaningful gene expression data. Obtaining intact fungal RNA from complex lignocellulosic substrates is often difficult, producing low integrity RNA which perform poorly in downstream applications. In this study we developed an RNA extraction method using CsCl centrifugation procedure, modified from previous reports and adapted for isolation of RNA from plant biomass. This method provided high level of integrity and good quantity of RNA which were suitable for reliable analyses of gene expression and produced consistent and reproducible results. Copyright © 2014 Elsevier Inc. All rights reserved.

Citation

Aleksandrina Patyshakuliyeva, Miia R Mäkelä, Outi-Maaria Sietiö, Ronald P de Vries, Kristiina S Hildén. An improved and reproducible protocol for the extraction of high quality fungal RNA from plant biomass substrates. Fungal genetics and biology : FG & B. 2014 Nov;72:201-6