Pyrophosphate selective fluorescent chemosensors: cascade recognition of nuclear stain mimicking DAPI.

A new zinc(ii) complex with a condensed hydroxynaphthyl pyridine (SPHN) as the coordinated ligand has been synthesized for the selective recognition of pyrophosphate (PPi) over other anions including phosphate in a mixed aqueous solution. The fluorescence enhancement of SPHN in association with Zn(2+) ions is quenched in the presence of intracellular pyrophosphate. This phenomenon is utilized in the construction of a logic gate. The binding of SPHN with Zn(2+) and its displacement by PPi have been established by photophysical investigation and supported by the DFT level of studies. The development of blue fluorescence in the {} complex upon binding of zinc with is shown to be useful as a nucleus marker in a cell similar to the commercially available staining compound, DAPI (diamino-2-phenylindole).

Citation

Shyamaprosad Goswami, Avijit Kumar Das, Bholanath Pakhira, Sohini Basu Roy, Anup Kumar Maity, Partha Saha, Sabyasachi Sarkar. Pyrophosphate selective fluorescent chemosensors: cascade recognition of nuclear stain mimicking DAPI. Dalton transactions (Cambridge, England : 2003). 2014 Sep 7;43(33):12689-97

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PMID: 25010909

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