Clear Search sequence regions


  • ATP (6)
  • atpases (2)
  • ceramides (2)
  • complex v (7)
  • drosophila (5)
  • drosophila proteins (2)
  • Gln (1)
  • human (2)
  • models molecular (1)
  • proton (2)
  • regulates (1)
  • Sirt2 (6)
  • SIRT3 (1)
  • sirtuin (3)
  • sirtuin 3 (3)
  • sphingolipid (1)
  • subunit (1)
  • Sizes of these terms reflect their relevance to your search.

    Adenosine triphosphate (ATP) synthase β, the catalytic subunit of mitochondrial complex V, synthesizes ATP. We show that ATP synthase β is deacetylated by a human nicotinamide adenine dinucleotide (NAD(+))-dependent protein deacetylase, sirtuin 3, and its Drosophila melanogaster homologue, dSirt2. dsirt2 mutant flies displayed increased acetylation of specific Lys residues in ATP synthase β and decreased complex V activity. Overexpression of dSirt2 increased complex V activity. Substitution of Lys 259 and Lys 480 with Arg in human ATP synthase β, mimicking deacetylation, increased complex V activity, whereas substitution with Gln, mimicking acetylation, decreased activity. Mass spectrometry and proteomic experiments from wild-type and dsirt2 mitochondria identified the Drosophila mitochondrial acetylome and revealed dSirt2 as an important regulator of mitochondrial energy metabolism. Additionally, we unravel a ceramide-NAD(+)-sirtuin axis wherein increased ceramide, a sphingolipid known to induce stress responses, resulted in depletion of NAD(+) and consequent decrease in sirtuin activity. These results provide insight into sirtuin-mediated regulation of complex V and reveal a novel link between ceramide and Drosophila acetylome. © 2014 Rahman et al.

    Citation

    Motiur Rahman, Niraj K Nirala, Alka Singh, Lihua Julie Zhu, Kaori Taguchi, Takeshi Bamba, Eiichiro Fukusaki, Leslie M Shaw, David G Lambright, Jairaj K Acharya, Usha R Acharya. Drosophila Sirt2/mammalian SIRT3 deacetylates ATP synthase β and regulates complex V activity. The Journal of cell biology. 2014 Jul 21;206(2):289-305

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 25023514

    View Full Text