Vanadate influence on metabolism of sugar phosphates in fungus Phycomyces blakesleeanus.

Milan Žižić, Miroslav Živić, Vuk Maksimović, Marina Stanić, Strahinja Križak, Tijana Cvetić Antić, Joanna Zakrzewska

PloS one 2014

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The biological and chemical basis of vanadium action in fungi is relatively poorly understood. In the present study, we investigate the influence of vanadate (V5+) on phosphate metabolism of Phycomyces blakesleeanus. Addition of V5+ caused increase of sugar phosphates signal intensities in 31P NMR spectra in vivo. HPLC analysis of mycelial phosphate extracts demonstrated increased concentrations of glucose 6 phosphate, fructose 6 phosphate, fructose 1, 6 phosphate and glucose 1 phosphate after V5+ treatment. Influence of V5+ on the levels of fructose 2, 6 phosphate, glucosamine 6 phosphate and glucose 1, 6 phosphate (HPLC), and polyphosphates, UDPG and ATP (31P NMR) was also established. Increase of sugar phosphates content was not observed after addition of vanadyl (V4+), indicating that only vanadate influences its metabolism. Obtained results from in vivo experiments indicate catalytic/inhibitory vanadate action on enzymes involved in reactions of glycolysis and glycogenesis i.e., phosphoglucomutase, phosphofructokinase and glycogen phosphorylase in filamentous fungi.

Citation

Milan Žižić, Miroslav Živić, Vuk Maksimović, Marina Stanić, Strahinja Križak, Tijana Cvetić Antić, Joanna Zakrzewska. Vanadate influence on metabolism of sugar phosphates in fungus Phycomyces blakesleeanus. PloS one. 2014;9(7):e102849