Correlation Engine 2.0
Clear Search sequence regions


  • calcium (2)
  • calmodulin (8)
  • CaMKII (5)
  • cells (1)
  • clamp (2)
  • dependent (4)
  • female (1)
  • kn 62 (3)
  • male (1)
  • N OFQ (7)
  • nociceptin (1)
  • nociceptin (4)
  • opioid peptides (2)
  • patch clamp techniques (1)
  • potassium (2)
  • Potassium Channels (2)
  • rat (2)
  • rats wistar (1)
  • w 7 (3)
  • Sizes of these terms reflect their relevance to your search.

    The neuropeptide nociceptin/orphanin FQ (N/OFQ) has been shown to inhibit delayed rectifier potassium current (IK) in acutely dissociated rat parietal cortical neurons. However, the detailed mechanism of N/OFQ-induced inhibition on IK is not clear. This study is the first to explore an involvement of calcium/calmodulin (CaM)-dependent protein kinase type II (CaMKII) in mediating N/OFQ-induced responses. Utilizing pharmacological inhibitors of CaM and CaMKII, we have investigated the contribution of CaMKII in N/OFQ-induced effects using the whole-cell patch-clamp technique. In whole-cell voltage clamp, W-7 (100 μM), an antagonist of CaM, as well as KN-62, an inhibitor of CaMKII activity, attenuated the inhibitory effects of N/OFQ on IK. Activation and inactivation analysis indicated that the kinetics of IK were altered by N/OFQ, with decreased activation and promoted inactivation of IK. W-7 and KN-62 (10 μM) partly abolished the activation and inactivation curves shift of IK induced by N/OFQ. These findings show that CaMKII plays a critical role in N/OFQ-induced inhibition of IK in acutely dissociated rat parietal cortical neurons.

    Citation

    Yanli Wang, Weidong Yue, Wei Wang, Juan Yu, Ruxia Liu, Peng Wang, Yonggang Cao, Qianlong Zhang, Xiaoyan Wang, Lihui Qu. Nociceptin/orphanin FQ-induced inhibition of delayed rectifier potassium currents by calcium/calmodulin-dependent protein kinase type II. Neuroreport. 2014 Oct 22;25(15):1227-31

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 25171201

    View Full Text