Structure and gene cluster of the O-antigen of Escherichia coli O68.

Lingyan Jiang, Andrei V Perepelov, Andrei V Filatov, Bin Liu, Alexander S Shashkov, Sof'ya N Senchenkova, Lei Wang, Yuriy A Knirel

Carbohydrate research 2014 Oct 9

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The O-polysaccharide (O-antigen) of Escherichia coli O68 was studied by sugar analysis, partial solvolysis with anhydrous trifluoroacetic acid, and 1D and 2D (1)H and (13)C NMR spectroscopies. The following structure of the branched heptasaccharide repeating unit was established: [structure: see text]. The O-antigen gene cluster of E. coli O68 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in full agreement with the O-antigen structure. Copyright © 2014 Elsevier Ltd. All rights reserved.

Citation

Lingyan Jiang, Andrei V Perepelov, Andrei V Filatov, Bin Liu, Alexander S Shashkov, Sof'ya N Senchenkova, Lei Wang, Yuriy A Knirel. Structure and gene cluster of the O-antigen of Escherichia coli O68. Carbohydrate research. 2014 Oct 9;397:27-30