Anthony Le Cigne, Vanessa Menil-Philippot, Fabrice Fleury, Masayuki Takahashi, Christophe Thiriet
Genes to cells : devoted to molecular & cellular mechanisms 2014 OctThe homologous recombination factor RAD51 is highly conserved. This criterion enabled us to identify a RAD51 ortholog in Physarum polycephalum. We found that the Physarum protein presents a high homology to the human protein and cross-reacted with antibodies directed against the human RAD51. Taking advantage of the natural synchrony of millions of nuclei within a single cell of Physarum, we investigated the fluctuation of the amount of the PpRAD51 throughout the cell cycle. Our results showed that in the late G2-phase, RAD51 was transiently expressed in a large quantity. Furthermore, knocking-down RAD51 in the G2-phase abolished this transient expression before mitosis and affected cell cycle progression. These results support the idea that RAD51 plays a role in the progression of the cell cycle in the late G2-phase. © 2014 The Authors Genes to Cells © 2014 by the Molecular Biology Society of Japan and Wiley Publishing Asia Pty Ltd.
Anthony Le Cigne, Vanessa Menil-Philippot, Fabrice Fleury, Masayuki Takahashi, Christophe Thiriet. Transient expression of RAD51 in the late G2-phase is required for cell cycle progression in synchronous Physarum cells. Genes to cells : devoted to molecular & cellular mechanisms. 2014 Oct;19(10):755-65
PMID: 25200281
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