Transcriptional response to the [ISP(+) ] prion of Saccharomyces cerevisiae differs from that induced by the deletion of its structural gene, SFP1.

Polina Drozdova, Tatyana Rogoza, Elina Radchenko, Polina Lipaeva, Ludmila Mironova

FEMS yeast research 2014 Dec

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Currently, several protein-based genetic determinants, or prions, are described in yeast, and several hundred prion candidates have been predicted. Importantly, many known and potential prion proteins regulate transcription; therefore, prion induction should affect gene expression. While it is generally believed that the prion phenotype should mimic the deletion phenotype, this rule has exceptions. Formed by the transcription factor Sfp1p, [ISP(+) ] is one such exception as the [ISP(+) ] and sfp1Δ strains differ in many phenotypic traits. These data suggest that effects of prion formation by a transcription factor and its absence may affect gene expression in a different way. However, studies addressing this issue are practically absent. Here, we explore how [ISP(+) ] affects gene expression and how these changes correspond to the effect of SFP1 deletion. Our data indicate that the [ISP(+) ]-related expression changes cannot be explained by the inactivation of Sfp1p. Remarkably, most Sfp1p targets are not affected in the [ISP(+) ] strain; instead, the genes upregulated in the [ISP(+) ] strain are enriched in Gcn4p and Aft1p targets. We propose that Sfp1p serves as a part of a regulatory complex, and the activity of this complex may be modulated differently by the absence or prionization of Sfp1p. © 2014 Federation of European Microbiological Societies. Published by John Wiley & Sons Ltd. All rights reserved.

Citation

Polina Drozdova, Tatyana Rogoza, Elina Radchenko, Polina Lipaeva, Ludmila Mironova. Transcriptional response to the [ISP(+) ] prion of Saccharomyces cerevisiae differs from that induced by the deletion of its structural gene, SFP1. FEMS yeast research. 2014 Dec;14(8):1160-70