Functional expression of recombinant human macrophage β-glucan receptor dectin-1 using baculovirus-silkworm expression system.

Minoru Ujita, Shota Koike, Yosuke Yamauchi, Noburo Kiochi, Hiroki Yura, Mayumi Yamanaka, Hiroki Okumura

Bioscience, biotechnology, and biochemistry 2014

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Human macrophage dectin-1, a type II transmembrane β-glucan receptor, was expressed as a fusion protein with an N-terminal hexahistidine tag in a baculovirus-silkworm expression system and assayed for binding activity. Recombinant dectin-1 specifically bound to some β-glucans, and the neck domain and N-linked oligosaccharide chains of human dectin-1 did not affect the ligand binding activity and specificity of the receptor.

Citation

Minoru Ujita, Shota Koike, Yosuke Yamauchi, Noburo Kiochi, Hiroki Yura, Mayumi Yamanaka, Hiroki Okumura. Functional expression of recombinant human macrophage β-glucan receptor dectin-1 using baculovirus-silkworm expression system. Bioscience, biotechnology, and biochemistry. 2014;78(7):1203-5