Assessment of ALA-induced PpIX production in porcine skin pretreated with microneedles.

Phamilla Gracielli Sousa Rodrigues, Priscila Fernanda Campos de Menezes, Alessandra Keiko Lima Fujita, André Escobar, Andrigo Barboza de Nardi, Cristina Kurachi, Vanderlei S Bagnato

Journal of biophotonics 2015 Sep

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Photodynamic therapy (PDT) is used for skin treatments of premalignant and cancer lesions and recognized as a non-invasive technique that combines tissue photosensitization and subsequent exposure to light to induce cell death. However, it is limited to the treatment of superficial lesions, mainly due to the low cream penetration. Therefore, the improvement of transdermal distribution of aminolevulinic acid (ALA) is needed. In this study, the kinetics and homogeneity of production of ALA-induced PpIX after the skin pre-treatment with microneedles rollers of 0.5, 1.0 and 1.5 mm length were investigated. An improvement in homogeneity and production of PpIX was shown in a porcine model. Widefield fluorescence imaging three hours after the topical application of ALA-cream in the combined treatment with microeedles rollers. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Citation

Phamilla Gracielli Sousa Rodrigues, Priscila Fernanda Campos de Menezes, Alessandra Keiko Lima Fujita, André Escobar, Andrigo Barboza de Nardi, Cristina Kurachi, Vanderlei S Bagnato. Assessment of ALA-induced PpIX production in porcine skin pretreated with microneedles. Journal of biophotonics. 2015 Sep;8(9):723-9