Heparan sulfate modulates Slit3-induced endothelial cell migration.

Hong Qiu, Wenyuan Xiao, Jingwen Yue, Lianchun Wang

Methods in molecular biology (Clifton, N.J.) 2015

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Heparan sulfate is a long, linear polysaccharide with sulfation modifications and belongs to the glycosaminoglycan family. Our recent studies elucidated that the axon guidance molecule Slit3 is a new heparan sulfate-binding protein and a novel angiogenic factor by interacting with its cognate receptor Robo4, which is specifically expressed in endothelial cells. Here we describe using heparan sulfate-deficient mouse endothelial cells to determine the co-reception function of heparan sulfate in Slit3-induced endothelial cell migration in a Boyden chamber trans-well migration assay.

Citation

Hong Qiu, Wenyuan Xiao, Jingwen Yue, Lianchun Wang. Heparan sulfate modulates Slit3-induced endothelial cell migration. Methods in molecular biology (Clifton, N.J.). 2015;1229:549-55