Evaluation of the expression of P-glycoprotein in propoxur-resistant Caco-2 cells.

There is a great concern about the effect of propoxur, as one of the more common N-methyl carbamate pesticides, on human health due to its extensive use in agricultural and non-agricultural applications. Caco-2 cells became resistant to propoxur, and the resistance was confirmed through MTT assay. Then the cell membrane integrity and P-glycoprotein expression were measured by LDH assay and western blot analysis, respectively and compared to the parent cells.  Contrary to what was expected, the expression of P-glycoprotein in propoxur resistant cells was lower than parent cells. This study indicates that the resistance to propoxur may not be related to P-glycoprotein expression directly, since P-glycoprotein expression has decreased in these cells.

Citation

Shabnam Yazdian, Najmeh Fahham, Mohammad Hossein Ghahremani, Monireh Afzali, Narges Farsandaj, Melody Vatankhah, Seyed Nasser Ostad. Evaluation of the expression of P-glycoprotein in propoxur-resistant Caco-2 cells. Acta medica Iranica. 2014;52(10):728-33

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PMID: 25369005

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