Development of a quantitative ELISA kit for human secreted CD306/LAIR-2 and its application.

Yuan Tao, Jun Lin, You-Jia Hu, Bin Zhou, Bao-Quan Zhu

Chinese journal of natural medicines 2014 Nov

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A quantitative ELISA kit for the detection of human secreted CD306/LAIR-2 was developed using monoclonal and polyclonal antibodies which were raised against a highly purified recombinant human secreted CD306/LAIR-2. Anti-human secreted CD306/LAIR-2 monoclonal and polyclonal antibodies were raised by immunizing mouse or rabbit with recombinant human secreted CD306/LAIR-2. The monoclonal antibody was purified by protein G affinity, whereas the polyclonal antibody was purified by protein A affinity. The best match pair of antibodies were found and used to develop a double antibody sandwich ELISA kit for the detection of human secreted CD306/LAIR-2 in human samples. A human secreted CD306/LAIR-2 ELISA kit was formulated with highly purified recombinant human secreted CD306/LAIR-2, highly specific monoclonal and polyclonal antibodies. This kit realized the quantitative measurement of recombinant human CD306/LAIR-2 and natural CD306/LAIR-2 in human serum samples. The developed human secreted CD306/LAIR-2 ELISA kit is a reliable quantitation immunoassay kit. Copyright © 2014 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.

Citation

Yuan Tao, Jun Lin, You-Jia Hu, Bin Zhou, Bao-Quan Zhu. Development of a quantitative ELISA kit for human secreted CD306/LAIR-2 and its application. Chinese journal of natural medicines. 2014 Nov;12(11):847-52