Dynamics on human Toll-like receptor 4 complexation to MD-2: the coreceptor stabilizing function.

Carla de Aguiar, Mauricio G S Costa, Hugo Verli

Proteins 2015 Feb

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The interaction between human Toll-like receptor 4 (hTLR4) and its coreceptor, myeloid differentiation factor 2 (MD-2), is important in Gram-negative bacteria lipopolysaccharide (LPS) recognition. In this process, MD-2 recognizes LPS and promotes the dimerization of the complex hTLR4-MD-2-LPS, triggering an intracellular immune signaling. In this study, we employed distinct computational methods to explore the dynamical properties of the hTLR4-MD-2 complex and investigated the implications of the coreceptor complexation to the structural biology of hTLR4. We characterized both global and local dynamics of free and MD-2 complexed hTLR4, in both (hTLR4-MD-2)1 and (hTLR4-MD-2)2 states. Both molecular dynamics and normal mode analysis reveled a stabilization of the terminal regions of hTLR4 upon complexation to MD-2. We are able to identify conserved important residues involved on the hTLR4-MD-2 interaction dynamics and disclose C-terminal motions that may be associated to the signaling process upon oligomerization. © 2014 Wiley Periodicals, Inc.

Citation

Carla de Aguiar, Mauricio G S Costa, Hugo Verli. Dynamics on human Toll-like receptor 4 complexation to MD-2: the coreceptor stabilizing function. Proteins. 2015 Feb;83(2):373-82