Cdc6 localizes to S- and G2-phase centrosomes in a cell cycle-dependent manner.

Gwang Su Kim, Jeeheon Kang, Sung Woong Bang, Deog Su Hwang

Biochemical and biophysical research communications 2015 Jan 16

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The Cdc6 protein has been primarily investigated as a component of the pre-replicative complex for the initiation of chromosome replication, which contributes to maintenance of chromosomal integrity. Here, we show that Cdc6 localized to the centrosomes during S and G2 phases of the cell cycle. The centrosomal localization was mediated by Cdc6 amino acid residues 311-366, which are conserved within other Cdc6 homologues and contains a putative nuclear export signal. Deletions or substitutions of the amino acid residues did not allow the proteins to localize to centrosomes. In contrast, DsRed tag fused to the amino acid residues localized to centrosomes. These results indicated that a centrosome localization signal is contained within amino acid residues 311-366. The cell cycle-dependent centrosomal localization of Cdc6 in S and G2 phases suggest a novel function of Cdc6 in centrosomes. Copyright © 2014 Elsevier Inc. All rights reserved.

Citation

Gwang Su Kim, Jeeheon Kang, Sung Woong Bang, Deog Su Hwang. Cdc6 localizes to S- and G2-phase centrosomes in a cell cycle-dependent manner. Biochemical and biophysical research communications. 2015 Jan 16;456(3):763-7