Bactobolin A binds to a site on the 70S ribosome distinct from previously seen antibiotics.

Alexey Amunts, Karol Fiedorczuk, Thao T Truong, Josephine Chandler, E Peter Greenberg, V Ramakrishnan

Journal of molecular biology 2015 Feb 27

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The ribosome is the target of a large number of antibiotics. Here, we report a 3.4-Å-resolution crystal structure of bactobolin A bound to 70S ribosome-tRNA complex. The antibiotic binds at a previously unseen site in the 50S subunit and displaces tRNA bound at the P-site. It thus likely has a similar mechanism of action as blasticidin S despite binding to a different site. The structure also rationalizes previously identified resistance mutations. Copyright © 2014. Published by Elsevier Ltd.

Citation

Alexey Amunts, Karol Fiedorczuk, Thao T Truong, Josephine Chandler, E Peter Greenberg, V Ramakrishnan. Bactobolin A binds to a site on the 70S ribosome distinct from previously seen antibiotics. Journal of molecular biology. 2015 Feb 27;427(4):753-5