Enhanced poly(3-hydroxybutyrate) production in transgenic tobacco BY-2 cells using engineered acetoacetyl-CoA reductase.

Toshinori Yokoo, Ken'ichiro Matsumoto, Takashi Ooba, Kenjiro Morimoto, Seiichi Taguchi

Bioscience, biotechnology, and biochemistry 2015

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Highly active mutant of NADPH-dependent acetoacetyl-CoA reductase (PhaB) was expressed in Nicotiana tabacum cv. Bright Yellow-2 cultured cells to produce poly(3-hydroxybutyrate) [P(3HB)]. The mutated PhaB increased P(3HB) content by three-fold over the control, indicating that the mutant was a versatile tool for P(3HB) production. Additionally, the PhaB-catalyzed reaction was suggested to be a rate-limiting step of P(3HB) biosynthesis in tobacco BY-2 cells.

Citation

Toshinori Yokoo, Ken'ichiro Matsumoto, Takashi Ooba, Kenjiro Morimoto, Seiichi Taguchi. Enhanced poly(3-hydroxybutyrate) production in transgenic tobacco BY-2 cells using engineered acetoacetyl-CoA reductase. Bioscience, biotechnology, and biochemistry. 2015;79(6):986-8