Tunable release of clavam from clavam stabilized gold nanoparticles--design, characterization and antimicrobial study.

V Manju, P Dhandapani, M Gurusamy Neelavannan, S Maruthamuthu, S Berchmans, A Palaniappan

Materials science & engineering. C, Materials for biological applications 2015 Apr

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A facile one-step approach is developed to synthesize highly stable (up to 6months) gold nanoparticles (GNPs) using Clavam, pharmaceutical form of amoxicillin which contains a mixture of amoxicillin and potassium salt of clavulanic acid, at room temperature (25-30°C). The clavam stabilized GNPs are characterized using various techniques including UV-Visible, FT-IR spectrophotometry and transmission electron microscopy (TEM). Tunable release of clavam from clavam stabilized GNPs is demonstrated using intracellular concentrations of glutathione (GSH). The process is monitored using an UV-Vis spectroscopy and the amount of clavam released in terms of amoxicillin concentration is quantitatively estimated using reverse phase high performance liquid chromatographic (RP-HPLC) technique. In vitro study reveals that the clavam released from GNPs' surface was found to show a significant enhancement in antibacterial activity against Escherichia coli and the cause of enhancement is addressed. Copyright © 2015 Elsevier B.V. All rights reserved.

Citation

V Manju, P Dhandapani, M Gurusamy Neelavannan, S Maruthamuthu, S Berchmans, A Palaniappan. Tunable release of clavam from clavam stabilized gold nanoparticles--design, characterization and antimicrobial study. Materials science & engineering. C, Materials for biological applications. 2015 Apr;49:500-8