Crystallization and preliminary crystallographic analysis of acetophenone reductase from Geotrichum candidum NBRC 4597.

Acetophenone reductase (APRD) from Geotrichum candidium NBRC 4597 was crystallized by the hanging-drop vapour-diffusion method using PEG 3350 as a precipitant. The crystal belonged to space group P6522, with unit-cell parameters a = b = 104.5, c = 273.7 Å, and diffracted to 2.6 Å resolution. Phasing using the single-wavelength anomalous diffraction method was successful. Model building and crystallographic refinement are in progress.

Citation

Yosuke Sugiyama, Miki Senda, Toshiya Senda, Tomoko Matsuda. Crystallization and preliminary crystallographic analysis of acetophenone reductase from Geotrichum candidum NBRC 4597. Acta crystallographica. Section F, Structural biology communications. 2015 Mar;71(Pt 3):320-3

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PMID: 25760708

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