Bookmark
Forward
Assaying the interaction of the Rab guanine nucleotide exchange protein Sec2 with the upstream Rab, a downstream effector, and a phosphoinositide.
Danièle Stalder, Peter J Novick
Methods in molecular biology (Clifton, N.J.) 2015
Sizes of these terms reflect their relevance to your search.
Rabs are activated by guanine nucleotide exchange proteins, which are in turn controlled by complex regulatory mechanisms. Here we describe several different assays that have been used to delineate the mechanisms by which Sec2p, the exchange factor for the Rab Sec4p, is regulated. These assays assess the interaction of Sec2p with the upstream Rab, Ypt32p, a downstream Sec4p effector, Sec15p, and the lipid, phosphatidylinositol-4-phosphate.
Citation
Danièle Stalder, Peter J Novick.
Assaying the interaction of the Rab guanine nucleotide exchange protein Sec2 with the upstream Rab, a downstream effector, and a phosphoinositide.
Methods in molecular biology (Clifton, N.J.).
2015;1298:85-98
Mesh Tags
Guanine Nucleotide Exchange Factors
Immunoprecipitation
Phosphatidylinositol Phosphates
Protein Binding
Protein Interaction Mapping
Saccharomyces cerevisiae Proteins
Vesicular Transport Proteins
rab GTP-Binding Proteins
Substances
Guanine Nucleotide Exchange Factors
Phosphatidylinositol Phosphates
SEC15 protein, S cerevisiae
SEC2 protein, S cerevisiae
Saccharomyces cerevisiae Proteins
Vesicular Transport Proteins
phosphatidylinositol 4-phosphate
YPT32 protein, S cerevisiae
rab GTP-Binding Proteins
PMID: 25800834
View Full Text