Aina Gotoh, Toshihiko Katoh, Yuta Sugiyama, Shin Kurihara, Yuji Honda, Haruko Sakurama, Taiho Kambe, Hisashi Ashida, Motomitsu Kitaoka, Kenji Yamamoto, Takane Katayama
Carbohydrate research 2015 May 18We describe the novel substrate specificities of two independently evolved lacto-N-biosidases (LnbX and LnbB) towards the sugar chains of globo- and ganglio-series glycosphingolipids. LnbX, a non-classified member of the glycoside hydrolase family, isolated from Bifidobacterium longum subsp. longum, was shown to liberate galacto-N-biose (GNB: Galβ1-3GalNAc) and 2'-fucosyl GNB (a type-4 trisaccharide) from Gb5 pentasaccharide and globo H hexasaccharide, respectively. LnbB, a member of the glycoside hydrolase family 20 isolated from Bifidobacterium bifidum, was shown to release GNB from Gb5 and GA1 oligosaccharides. This is the first report describing enzymatic release of β-linked GNB from natural substrates. These unique activities may play a role in modulating the microbial composition in the gut ecosystem, and may serve as new tools for elucidating the functions of sugar chains of glycosphingolipids. Copyright © 2015 Elsevier Ltd. All rights reserved.
Aina Gotoh, Toshihiko Katoh, Yuta Sugiyama, Shin Kurihara, Yuji Honda, Haruko Sakurama, Taiho Kambe, Hisashi Ashida, Motomitsu Kitaoka, Kenji Yamamoto, Takane Katayama. Novel substrate specificities of two lacto-N-biosidases towards β-linked galacto-N-biose-containing oligosaccharides of globo H, Gb5, and GA1. Carbohydrate research. 2015 May 18;408:18-24
PMID: 25839135
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