Cyanohydrin reactions enhance glycolytic oscillations in yeast.

Bjørn Olav Hald, Astrid Gram Nielsen, Christian Tortzen, Preben Graae Sørensen

Biophysical chemistry 2015 May-Jun

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Synchronous metabolic oscillations can be induced in yeast by addition of glucose and removal of extracellular acetaldehyde (ACAx). Compared to other means of ACAx removal, cyanide robustly induces oscillations, indicating additional cyanide reactions besides ACA to lactonitrile conversion. Here, (13)C NMR is used to confirm our previous hypothesis, that cyanide directly affects glycolytic fluxes through reaction with carbonyl-containing compounds. Intracellularly, at least 3 cyanohydrins were identified. Extracellularly, all signals could be identified and lactonitrile was found to account for ~66% of total cyanide removal. Simulations of our updated computational model show that intracellular cyanide reactions increase the amplitude of oscillations and that cyanide addition lowers [ACA] instantaneously. We conclude that cyanide provides the following means of inducing global oscillations: a) by reducing [ACAx] relative to oscillation amplitude, b) by targeting multiple intracellular carbonyl compounds during fermentation, and c) by acting as a phase resetting stimulus. Copyright © 2015 Elsevier B.V. All rights reserved.

Citation

Bjørn Olav Hald, Astrid Gram Nielsen, Christian Tortzen, Preben Graae Sørensen. Cyanohydrin reactions enhance glycolytic oscillations in yeast. Biophysical chemistry. 2015 May-Jun;200-201:18-26