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ZG16p is a soluble mammalian lectin that interacts with mannose and heparan sulfate. Here we describe detailed analysis of the interaction of human ZG16p with mycobacterial phosphatidylinositol mannosides (PIMs) by glycan microarray and NMR. Pathogen-related glycan microarray analysis identified phosphatidylinositol mono- and di-mannosides (PIM1 and PIM2) as novel ligand candidates of ZG16p. Saturation transfer difference (STD) NMR and transferred NOE experiments with chemically synthesized PIM glycans indicate that PIMs preferentially interact with ZG16p by using the mannose residues. The binding site of PIM was identified by chemical-shift perturbation experiments with uniformly (15)N-labeled ZG16p. NMR results with docking simulations suggest a binding mode of ZG16p and PIM glycan; this will help to elucidate the physiological role of ZG16p. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Shinya Hanashima, Sebastian Götze, Yan Liu, Akemi Ikeda, Kyoko Kojima-Aikawa, Naoyuki Taniguchi, Daniel Varón Silva, Ten Feizi, Peter H Seeberger, Yoshiki Yamaguchi. Defining the Interaction of Human Soluble Lectin ZG16p and Mycobacterial Phosphatidylinositol Mannosides. Chembiochem : a European journal of chemical biology. 2015 Jul 6;16(10):1502-11

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PMID: 25919894

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