Anti-dormant mycobacterial activity and target analysis of nybomycin produced by a marine-derived Streptomyces sp.

Masayoshi Arai, Kentaro Kamiya, Patamaporn Pruksakorn, Yuji Sumii, Naoyuki Kotoku, Jean-Pierre Joubert, Prashini Moodley, Chisu Han, Dayoung Shin, Motomasa Kobayashi

Bioorganic & medicinal chemistry 2015 Jul 1

filter terms:

In the course of our search for anti-dormant Mycobacterial substances, nybomycin (1) was re-discovered from the culture broth of a marine-derived Streptomyces sp. on the bioassay-guided separation. Compound 1 showed anti-microbial activity against Mycobacterium smegmatis and Mycobacterium bovis BCG with the MIC of 1.0μg/mL under both actively growing aerobic conditions and dormancy inducing hypoxic conditions. Compound 1 is also effective to Mycobacterium tuberculosis including the clinically isolated strains. The mechanistic analysis indicated that 1 bound to DNA and induces a unique morphological change to mycobacterial bacilli leading the bacterial cell death. Copyright © 2015 Elsevier Ltd. All rights reserved.

Citation

Masayoshi Arai, Kentaro Kamiya, Patamaporn Pruksakorn, Yuji Sumii, Naoyuki Kotoku, Jean-Pierre Joubert, Prashini Moodley, Chisu Han, Dayoung Shin, Motomasa Kobayashi. Anti-dormant mycobacterial activity and target analysis of nybomycin produced by a marine-derived Streptomyces sp. Bioorganic & medicinal chemistry. 2015 Jul 1;23(13):3534-41