Elodie Reynaud, Liza L Lahaye, Ana Boulanger, Iveta M Petrova, Claire Marquilly, Adrien Flandre, Tania Martianez, Martin Privat, Jasprina N Noordermeer, Lee G Fradkin, Jean-Maurice Dura
Cell reports 2015 May 26In vivo axon pathfinding mechanisms in the neuron-dense brain remain relatively poorly characterized. We study the Drosophila mushroom body (MB) axons, whose α and β branches connect to different brain areas. We show that the Ryk family WNT5 receptor, DRL (derailed), which is expressed in the dorsomedial lineages, brain structure precursors adjacent to the MBs, is required for MB α branch axon guidance. DRL acts to capture and present WNT5 to MB axons rather than transduce a WNT5 signal. DRL's ectodomain must be cleaved and shed to guide α axons. DRL-2, another Ryk, is expressed within MB axons and functions as a repulsive WNT5 signaling receptor. Finally, our biochemical data support the existence of a ternary complex composed of the cleaved DRL ectodomain, WNT5, and DRL-2. Thus, the interaction of MB-extrinsic and -intrinsic Ryks via their common ligand acts to guide MB α axons. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.
Elodie Reynaud, Liza L Lahaye, Ana Boulanger, Iveta M Petrova, Claire Marquilly, Adrien Flandre, Tania Martianez, Martin Privat, Jasprina N Noordermeer, Lee G Fradkin, Jean-Maurice Dura. Guidance of Drosophila Mushroom Body Axons Depends upon DRL-Wnt Receptor Cleavage in the Brain Dorsomedial Lineage Precursors. Cell reports. 2015 May 26;11(8):1293-304
PMID: 25981040
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