Bax and Bif-1 proteins interact on Bilayer Lipid Membrane and form pore.

Rajeev Gupta, Subhendu Ghosh

Biochemical and biophysical research communications 2015 Aug 7

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Bax and Bax interacting factor-1(Bif-1) are cytosolic proteins, which translocate towards mitochondria during mitochondria-mediated apoptosis. Bif-1 has been identified to co-immunoprecipitate with Bax in apoptotic cells. We have studied the interaction of Bax and Bif-1 on Bilayer Lipid Membrane (BLM) through electrophysiological experiments. It has been observed that Bax-Bif-1 equimolar mixture can form a pore. The pore conductance is in the range of 4.96-5.41 nS. It also displays a sub-state with a conductance of 2.6 nS. No pore activity is observed on BLM when monomeric Bax and Bif-1 proteins are tested independently. The above-mentioned pore forming activity could be relevant in mitochondria-mediated apoptosis. Copyright © 2015 Elsevier Inc. All rights reserved.

Citation

Rajeev Gupta, Subhendu Ghosh. Bax and Bif-1 proteins interact on Bilayer Lipid Membrane and form pore. Biochemical and biophysical research communications. 2015 Aug 7;463(4):751-5