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Fluorescence resonance energy transfer-based assay showed that archaeal ribonuclease P (RNase P) proteins significantly promoted DNA annealing and strand displacement. Moreover, we found that archaeal RNase P proteins could discriminate nucleotide exchanges in DNA chains via their activity accelerating DNA strand displacement, suggesting that they have potential for biotechnological application to genetic diagnosis.


Mitsuru Miyanoshita, Takashi Nakashima, Yoshimitsu Kakuta, Makoto Kimura. Archaeal ribonuclease P proteins have potential for biotechnological applications where precise hybridization of nucleic acids is needed. Bioscience, biotechnology, and biochemistry. 2015;79(12):2014-7

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PMID: 26114935

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