The Nuclear PolyA-Binding Protein Nab2p Is Essential for mRNA Production.

Manfred Schmid, Pawel Olszewski, Vicent Pelechano, Ishaan Gupta, Lars M Steinmetz, Torben Heick Jensen

Cell reports 2015 Jul 7

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Polyadenylation of mRNA is a key step in eukaryotic gene expression. However, despite the major impact of poly(A) tails on mRNA metabolism, the precise roles of poly(A)-binding proteins (PABPs) in nuclear mRNA biogenesis remain elusive. Here, we demonstrate that rapid nuclear depletion of the S. cerevisiae PABP Nab2p leads to a global loss of cellular mRNA, but not of RNA lacking poly(A) tails. Disappearance of mRNA is a nuclear event, but not due to decreased transcription. Instead, the absence of Nab2p results in robust nuclear mRNA decay by the ribonucleolytic RNA exosome in a polyadenylation-dependent process. We conclude that Nab2p is required to protect early mRNA and therefore constitutes a crucial nuclear mRNA biogenesis factor. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

Citation

Manfred Schmid, Pawel Olszewski, Vicent Pelechano, Ishaan Gupta, Lars M Steinmetz, Torben Heick Jensen. The Nuclear PolyA-Binding Protein Nab2p Is Essential for mRNA Production. Cell reports. 2015 Jul 7;12(1):128-39