Correlation Engine 2.0
Clear Search sequence regions

  • bacteria (1)
  • cdc2 protein (2)
  • cell cycle (2)
  • CKIs (1)
  • Cyclins (5)
  • humans (1)
  • plasmids (1)
  • subunits (1)
  • vitro (1)
  • Sizes of these terms reflect their relevance to your search.

    Cyclin-dependent kinases (Cdks) belong to a family of key regulators of cell division cycle and transcription. Their activity is mainly regulated by association with regulatory subunits named cyclins but their activities are also regulated by phosphorylation, acetylation, and the association with specific inhibitory proteins (CKIs). The activity of different Cdks is deregulated in many different type of tumors, and thus, Cdks are considered targets for antitumoral therapy. For large screenings of inhibitors the use of purified recombinant Cdks and cyclins is recommended. We report here the current methods to determine their in vitro activity for large screenings of inhibitors.


    Edurne Gallastegui, Oriol Bachs. Expression and Purification of Recombinant Cyclins and CDKs for Activity Evaluation. Methods in molecular biology (Clifton, N.J.). 2016;1336:9-12

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 26231704

    View Full Text