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A peptide sensor that integrates the 4-dimethylaminophthalimide (4-DMAP) fluorophore in a short cyclin A binding sequence displays a large fluorescence emission increase upon interacting with the cyclin A Binding Groove (CBG). Competitive displacement assays of this probe allow the straightforward identification of peptides that interact with the CBG, which could potentially block the recognition of CDK/cyclin A kinase substrates.


Elena Pazos, José L Mascareñas, M Eugenio Vázquez. Identification of Cyclin A Binders with a Fluorescent Peptide Sensor. Methods in molecular biology (Clifton, N.J.). 2016;1336:67-83

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PMID: 26231709

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