TRIzol and Alu qPCR-based quantification of metastatic seeding within the skeleton.

J Preston Campbell, P Mulcrone, S K Masood, M Karolak, A Merkel, K Hebron, A Zijlstra, J Sterling, F Elefteriou

Scientific reports 2015

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Current methods for detecting disseminated tumor cells in the skeleton are limited by expense and technical complexity. We describe a simple and inexpensive method to quantify, with single cell sensitivity, human metastatic cancer in the mouse skeleton, concurrently with host gene expression, using TRIzol-based DNA/RNA extraction and Alu sequence qPCR amplification. This approach enables precise quantification of tumor cells and corresponding host gene expression during metastatic colonization in xenograft models.

Citation

J Preston Campbell, P Mulcrone, S K Masood, M Karolak, A Merkel, K Hebron, A Zijlstra, J Sterling, F Elefteriou. TRIzol and Alu qPCR-based quantification of metastatic seeding within the skeleton. Scientific reports. 2015;5:12635