Delineation of BmSXP antibody V-gene usage from a lymphatic filariasis based immune scFv antibody library.

Phage display technology is an important tool for antibody generation or selection. This study describes the development of a scFv library and the subsequent analysis of identified monoclonal antibodies against BmSXP, a recombinant antigen for lymphatic filariasis. The immune library was generated from blood of lymphatic filariasis infected individuals. A TA based intermediary cloning approach was used to increase cloning efficiency for the library construction process. A diverse immune scFv library of 10(8) was generated. Six unique monoclonal antibodies were identified from the 50 isolated clones against BmSXP. Analysis of the clones showed a bias for the IgHV3 and Vκ1 (45.5%) and IgHV2 and Vκ3 (27.3%) gene family. The most favored J segment for light chain is IgKJ1 (45.5%). The most favored D and J segment for heavy chain are IgHD6-13 (75%) and IgHJ3 (47.7%). The information may suggest a predisposition of certain V genes in antibody responses against lymphatic filariasis. Copyright © 2015 Elsevier Ltd. All rights reserved.

Citation

Anizah Rahumatullah, Azimah Ahmad, Rahmah Noordin, Theam Soon Lim. Delineation of BmSXP antibody V-gene usage from a lymphatic filariasis based immune scFv antibody library. Molecular immunology. 2015 Oct;67(2 Pt B):512-23

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PMID: 26277276

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