Astrid Elisabeth Mork-Jansson, Daniela Gargano, Karol Kmiec, Clemens Furnes, Dmitriy Shevela, Lutz Andreas Eichacker
FEBS letters 2015 Oct 7The two-helix light harvesting like (Lil) protein Lil3 belongs to the family of chlorophyll binding light harvesting proteins of photosynthetic membranes. A function in tetrapyrrol synthesis and stabilization of geranylgeraniol reductase has been shown. Lil proteins contain the chlorophyll a/b-binding motif; however, binding of chlorophyll has not been demonstrated. We find that Lil3.2 from Arabidopsis thaliana forms heterodimers with Lil3.1 and binds chlorophyll. Lil3.2 heterodimerization (25±7.8 nM) is favored relative to homodimerization (431±59 nM). Interaction of Lil3.2 with chlorophyll a (231±49 nM) suggests that heterodimerization precedes binding of chlorophyll in Arabidopsis thaliana. Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Astrid Elisabeth Mork-Jansson, Daniela Gargano, Karol Kmiec, Clemens Furnes, Dmitriy Shevela, Lutz Andreas Eichacker. Lil3 dimerization and chlorophyll binding in Arabidopsis thaliana. FEBS letters. 2015 Oct 7;589(20 Pt B):3064-70
PMID: 26320415
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