Imaging Acute Neuromuscular Explants from Thy1 Mouse Lines.

Because core facilities that generate transgenic founder mice for a reasonable fee are now available at most major research institutions, generating new Thy1-XFP transgenic animals (in which XFP stands for any fluorescent protein) is an option even for relatively small laboratories. Here, we provide a protocol for screening offspring of Thy1 transgenic founders. Acute neuromuscular explants are obtained from 3-wk-old F1 mice that have been produced by crossing Thy1 transgenic founders and commercially obtained inbred mice. Thy1-driven expression is detected by fluorescence microscopy. © 2015 Cold Spring Harbor Laboratory Press.

Citation

Petar Marinković, Leanne Godinho, Thomas Misgeld. Imaging Acute Neuromuscular Explants from Thy1 Mouse Lines. Cold Spring Harbor protocols. 2015 Sep;2015(9):pdb.prot087692

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PMID: 26330628

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