Cytoplasmic Control of Sense-Antisense mRNA Pairs.

Flore Sinturel, Albertas Navickas, Maxime Wery, Marc Descrimes, Antonin Morillon, Claire Torchet, Lionel Benard

Cell reports 2015 Sep 22

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Transcriptome analyses have revealed that convergent gene transcription can produce many 3'-overlapping mRNAs in diverse organisms. Few studies have examined the fate of 3'-complementary mRNAs in double-stranded RNA-dependent nuclear phenomena, and nothing is known about the cytoplasmic destiny of 3'-overlapping messengers or their impact on gene expression. Here, we demonstrate that the complementary tails of 3'-overlapping mRNAs can interact in the cytoplasm and promote post-transcriptional regulatory events including no-go decay (NGD) in Saccharomyces cerevisiae. Genome-wide experiments confirm that these messenger-interacting mRNAs (mimRNAs) form RNA duplexes in wild-type cells and thus have potential roles in modulating the mRNA levels of their convergent gene pattern under different growth conditions. We show that the post-transcriptional fate of hundreds of mimRNAs is controlled by Xrn1, revealing the extent to which this conserved 5'-3' cytoplasmic exoribonuclease plays an unexpected but key role in the post-transcriptional control of convergent gene expression. Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.

Citation

Flore Sinturel, Albertas Navickas, Maxime Wery, Marc Descrimes, Antonin Morillon, Claire Torchet, Lionel Benard. Cytoplasmic Control of Sense-Antisense mRNA Pairs. Cell reports. 2015 Sep 22;12(11):1853-64