Electrochemical biosensor for Ni(2+) detection based on a DNAzyme-CdSe nanocomposite.

The detection and speciation analysis of metal-ion is very important for environmental monitoring. A novel electrochemical biosensor for Nickel(II) detection based on a DNAzyme-CdSe nanocomposite was developed. We firstly hybridized with capture probe (DNA1) and sequentially with DNA (DNA2) on the gold electrode. Then CdSe QDs were incorporated the specific recognition of DNA2 by covalent assembling. Upon addition of nickel ion into the above system, the substrate strand of the immobilized DNAzyme was catalytically cleaved by target Ni(2+), resulting in disassociation of the shorter DNA fragments containing CdSe QDs. The remaining CdSe QDs on the electrode surface detected by differential pulse anodic stripping voltammetry (DPASV). Under optimal conditions, the as-prepared sensor exhibited high sensitivity and fast response to Ni(2+) with the linear range from 20 nM to 0.2mM and a low detection limit of 6.67 nM. The prepared biosensor also shows good stability and good reproducibility and high selectivity toward target Ni(2+) against other metal ions because of highly specific Ni(2+)-dependent DNAzyme. Thus, our strategy has a good potential in the environment surveys. Copyright © 2015 Elsevier B.V. All rights reserved.

Citation

Ying Yang, Zheng Yuan, Xing-Pei Liu, Qiao Liu, Chang-Jie Mao, He-Lin Niu, Bao-Kang Jin, Sheng-Yi Zhang. Electrochemical biosensor for Ni(2+) detection based on a DNAzyme-CdSe nanocomposite. Biosensors & bioelectronics. 2016 Mar 15;77:13-8

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PMID: 26385732

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