Transmembrane prostatic acid phosphatase (TMPAP) delays cells in G1 phase of the cell cycle.

César L Araujo, Ileana B Quintero, Kristian Ovaska, Annakaisa M Herrala, Sampsa Hautaniemi, Pirkko T Vihko

The Prostate 2016 Feb

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Prostate adenocarcinoma is the most common form of prostate cancer. We have previously shown in a murine model that prostatic acid phosphatase (PAP) deficiency leads to increased cell proliferation and development of prostate adenocarcinoma. The association between PAP and prostate cancer has been reported. Indeed, high PAP enzymatic activity is detected in the serum of patients with metastatic disease while its expression is reduced in prostate cancer tissue. However, the molecular mechanisms behind the onset of the disease remains poorly understood. We previously identified a novel transmembrane prostatic acid phosphatase (TMPAP) isoform, which interacts with snapin. TMPAP is expressed on the plasma membrane, as well as endosomal/lysosomal and exosomal membrane vesicles by means of a tyrosine-based lysosomal targeting motif (Yxxϕ). We used stable overexpression of the secreted isoform (SPAP) and TMPAP in LNCaP cells, live cell imaging, microarray and qRT-PCR analyses, and fluid phase uptake of HRP and transferrin. Our results indicate that the stable overexpression of TMPAP, but not SPAP in LNCaP cells reduces cell growth while increasing endo/exocytosis and cell size. Specifically, cells overexpressing TMPAP accumulate in the G1 phase of the cell cycle, and show altered gene expression profile. Our data suggests that TMPAP may function as a non-canonical tumor suppressor by delaying cell growth in G1 phase of the cell cycle. © 2015 Wiley Periodicals, Inc.

Citation

César L Araujo, Ileana B Quintero, Kristian Ovaska, Annakaisa M Herrala, Sampsa Hautaniemi, Pirkko T Vihko. Transmembrane prostatic acid phosphatase (TMPAP) delays cells in G1 phase of the cell cycle. The Prostate. 2016 Feb;76(2):151-62